The CVI Protein Expression/Purification (PEP) Core offers a full range of protein expression and purification (PEP) services. Our primary mission is to provide services to UGA users, but we also offer our services to external users including non-UGA academic users and commercial companies.
Expression Service
The facility currently utilizes a mammalian cell expression system. We have a library of expression vectors that users have access to for their projects. Typically, users provide a sequence-verified expression vector containing the gene(s) of interest.
Mammalian Cell Expression
- • We grow and maintain three main suspension cell lines cultured in minimal media: EXPI293.
- • We have a number of adherent cell lines that are used for specialized circumstances like limiting glycosylation or enabling DHFR stable cell selection.
- • We are able to generate stable, clonal cell lines expressing your protein of interest. We also have several strategies that we can use to optimize expression levels in these systems.
- Small-scale test expressions in both suspension and adherent cultures via transient transfection can be conducted to determine optimal expression conditions.
- • We have the capacity to carry out large scale expression experiments (suspension and adherent), Max ~15L of suspension culture and ~3L of adherent culture in roller bottles.
- • We also have the expertise to generate, screen and produce murine monoclonal antibodies.
Protein Purification Service
The facility has all the relevant materials, instrumentation, and expertise to accommodate all popular purification methods and systems; from basic gravity columns and dialysis to high end FPLCs and Tangential flow filtration. If users need to utilize a technique and/or system that is not present in the facility we will work with you to try and get it working here.
• Fast Protein Liquid Chromatography (FPLC):
- The facility is equipped with FPLCs and a wide assortment of both high- and low-grade chromatography columns, covering the vast majority of protein-purification needs (affinity capture/ion exchange/gel filtration). In addition, users may supply their own columns for specialized projects.
• AKTA Pure:
- We have 1 of these instruments. They are advanced chromatography systems designed for automated, multistep protein purification of both single and multiple samples. The instrument is paired with UNICORN software that provides a user-friendly interface and customization of various purification methods. The core is also equipped with a roller bottle apparatus, enabling production of large quantities of monoclonal antibodies.
• Tangential Flow Filtration (TFF):
- TFF is a process for the rapid concentration and dia-filtration (buffer-exchange) of large volumes of solutions, typically in preparation for further purification steps. Our system can accommodate volumes up to 10L and proteins with molecular weights of 10KDa and larger.
| AKTA Protein Purification | CVI Investigators | Non-CVI Researchers | Industry |
|---|---|---|---|
| Consultation | $ 0 | $ 0 | $ 0 |
| Performance/hr | $25 | $50 | $50 |
| Supplies | at cost | at cost | at cost |
Researchers interested in using the Protein Expression and Purification Core service should contact Michael Carlock (mcarlock@uga.edu) or Michael Kulik (mkulik@uga.edu) to schedule a consultation.
For targeted protein purification solutions, see GE Targeted Solutions
For protein expression, see Expi293 and the Protein Expression Guide